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pCLL9
pCLL9
規(guī)格:
貨期:
編號:B226945
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 pCLL9
商品貨號 B226945
Designations pCLL9
Depositors American Cyanamid Co., JS Feitelson, American Cyanamid Co.
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Vector Information
Size (kb): 9.9099998474121090
Vector: pCLL9 (plasmid)
Construction: pCLL7, pIJ702
Marker(s):ampR,tsrR
Construct size (kb): 9.90999984741211
Features: insert detection: mel
marker(s): ampR
marker(s): tsrR
replicon: pAkij1
Applications
shuttle vector
vector permitting visual detection of recombinants tyrosinase
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--10.2; BglII--10.2; PstI--10.2.
Cloning into the PstI site inactivates the ampicillin marker used for selection in Escherichia coli.
Cloning into the BglII, SphI, or SstI sites inactivates the tyrosinase gene.
The pAkij1 ori may be useful in other taxa such as Bacillus, Staphylococcus and other Actinomycetes, including Streptosporangium, Actinoplanes, Nocardia, and Micromonospora.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Feitelson JS. Multifunctional plasmid vectors from Actinomadura and Escherichia coli. US Patent 5,002,891 dated Mar 26 1991

Feitelson JS, et al. Genetic and biochemical characterization of the red gene cluster of streptomyces coelicolor A3(2). J. Gen. Microbiol. 131: 2431-2441, 1985. PubMed: 2999302

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