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pGEM2MCSF10 [CMCC 2760]
pGEM2MCSF10 [CMCC 2760]
規(guī)格:
貨期:
編號:B227282
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pGEM2MCSF10 [CMCC 2760]
商品貨號 B227282
Designations pGEM2MCSF10 [CMCC 2760]
Species Mus musculus, mouse
Depositors JC Hunter-Cevera
Vector
Construct size (kb): 6.900000095367432
Insert
DNA: cDNA
Insert lengths(kb): 4.0
Gene product: colony stimulating factor, macrophage [Csfm]
Insert Size (kb) 4.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
The insert contains the following sites separated by (bp)(approximate): EcoRI- 310- PstI- 630- AvaI- 136- HindIII- 533- BamHI- 1390- HindIII- 932- EcoRI.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.85, 2.9; HindIII--3.8, 1.95, 0.95; PstI--3.65, 3.1; BamHI--4.35, 2.35; XbaI--6.8.
The insert is oriented in the sense direction relative to the SP6 promoter, such that RNA transcription will give translatable mRNA.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Ladner MB, et al. cDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells. Proc. Natl. Acad. Sci. USA 85: 6706-6710, 1988. PubMed: 2457916

Jennie C Hunter-Cevera, personal communication

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