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HD2G1
HD2G1
規(guī)格:
貨期:
編號:B228014
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 HD2G1
商品貨號 B228014
Designations HD2G1
GenBank Number

M29066

Species Homo sapiens, human
Depositors O Civelli, DK Grandy
Applications
Codominant inheritance was observed in 3 informative families (39 children).
Vector
Construct size (kb): 44.0
Insert
DNA: genomic
Insert lengths(kb): 16.0
Tissue: male
Gene product: dopamine receptor D2 [DRD2]
Alleles: A1, B1, B2, A2
Insert Size (kb) 16.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 9.0, 5.0, 4.3, 1.8, 1.7, 1.7, 0.7; BglII--25.0, 7.0, 3.2, 3.0, 2.4, 1.5, 0.68; PvuI--20.0, 12.0, 8.0; HindIII--21.0, 18.0, 4.3, 0.8; SmaI--20.0, 19.0, 5.6.
The insert contains the following sites separated by (kb)(approx): SalI, MboII- 1.5- BamHI- 1.7- BamHI- 1.7- BamHI- 4.3- BamHI- 1.8- BamHI- 0.65- BamHI- 5.0- SmaI- 0.6- BamHI- 0.7- MboII, SalI.
Codominant inheritance was observed in 3 informative families (39 children).
The 1.6 kb BamHI fragment contains exon 7 and 3' sequences. Exon 7, which has been sequenced, encodes the last 64 amino acids and includes 1.2 kb of 3' non-coding sequence.
The BamHI 1.6 doublet detects the TaqI RFLP associated with DRD2.
Use standard hybridization conditions with the addition of human placental DNA to block repeat sequences. Washing conditions are standard with a final wash at 0.1 x SSC, 0.1% SDS, 65C.
Enzyme(s) not detecting polymorphism: 31 enzymes.
References

Grandy DK, et al. The human dopamine D2 receptor gene is located on chromosome 11 at q22-q23 and identifies a TaqI RFLP. Am. J. Hum. Genet. 45: 778-785, 1989. PubMed: 2573278

Grandy DK, et al. Cloning of the cDNA and gene for a human D2 dopamine receptor. Proc. Natl. Acad. Sci. USA 86: 9762-9766, 1989. PubMed: 2532362

Olivier Civelli, personal communication

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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