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alpha1JN [alphaNJ, pJN I]
alpha1JN [alphaNJ, pJN I]
規(guī)格:
貨期:
編號(hào):B228432
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 alpha1JN [alphaNJ, pJN I]
商品貨號(hào) B228432
Designations alpha1JN [alphaNJ, pJN I]
GenBank Number

J00067

Species Homo sapiens, human
Depositors NA Kalsheker
Applications
Enzyme(s) not detecting polymorphism (for alpha1-antitrypsin gene-related sequence): BamHI, HindIII, MspI, PstI, XbaI, XhoII (tested against 20 unrelated Caucasians).
Vector
Construct size (kb): 4.0
Insert
DNA: cDNA
Insert lengths(kb): 0.25
Tissue: liver
Gene product: alpha-1-antitrypsin-like [PI]
Alleles: G3, A1, A2, B1, B2, D2, G1, G2, J2, N1, N2, C1, C2, D1, J1, A1, A2, D1, D2
Insert Size (kb) 0.25
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/AvaI--2.55, 1.45; PstI--4.0; EcoRI--4.0; AvaI--4.0; HindIII--4.0.
The sequence starts at amino acid 363 and continues through a poly(A) tail of 50 bp. The PstI site at the 3' end was not reconstituted.
The insert encompasses the last exon including the 3' untranslated region.
Codominant segregation for the EcoRI RFLP has been demonstrated in 2 informative families.
The gene-related sequence which demonstrates the EcoRI polymorphism maps 15 kb downstream from the alpha-1-antitrypsin gene.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
The clone is for alpha1-antitrypsin but can detect a related sequence. Signals can be distinguished by the sequence and the sizes of fragments generated. At higher stringency this can be made specific for alpha1-antitrypsin.
Enzyme(s) not detecting polymorphism (for alpha1-antitrypsin gene-related sequence): BamHI, HindIII, MspI, PstI, XbaI, XhoII (tested against 20 unrelated Caucasians).
References

Rogers J, et al. The isolation of a clone for human alpha 1-antitrypsin and the detection of alpha 1-antitrypsin in mRNA from liver and leukocytes. Biochem. Biophys. Res. Commun. 116: 375-382, 1983. PubMed: 6606425

Hodgson I, Kalsheker N. RFLP for a gene-related sequence of alpha 1-antitrypsin (AAT). Nucleic Acids Res. 14: 6779, 1986. PubMed: 3018681

Barbara Weiffenbach, personal communication

Noor A Kalsheker, personal communication

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