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pSH
pSH
規(guī)格:
貨期:
編號:B228481
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pSH
商品貨號 B228481
Designations pSH
Depositors AM Ventura
Biosafety Level 1
Vector Information
Size (kb): 5.9000000953674320
Vector: pSH (plasmid)
Promoters: Promoter T3
Construction: pEN1, BLpSV
Marker(s):ampR
Construct size (kb): 5.900000095367432
Features: marker(s): ampR
promoter: HTLV-1 LTR, T7, T3
replicon: pMB1, SV40
terminator: SV40 polyadenylation
enhancer: HIV-1 LTR
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.9; HindIII--5.9; PstI--4.3, 1.6.
Shuttle expression vector utilizing a chimeric promoter derived from the HTLV-I LTR and the HIV-1 LTR. Supports high levels of expression in human and mouse cells.
The order of the major features in the plasmid is: T7 promoter - XhoI - SV40 promoter - HTLV-1 LTR promoter - HIV-1 LTR enhancer - EcoRI/MCS/BglII - SV40 polyadenylation - T3 promoter - pMB1 ori - ampR.
Media ATCC® Medium 1946: Terrific broth (ATCC medium 1743) with 100 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Ventura AM, Villa LL. Plasmid pSH: a strong mammalian expression vector. Biochem. Biophys. Res. Commun. 192: 867-869, 1993. PubMed: 8387291

Shipped freeze-dried
Shipping Information Freeze dried plasmid in E. coli

梅經(jīng)理 17280875617 1438578920
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周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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