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phgH6 [hGH6]
phgH6 [hGH6]
規(guī)格:
貨期:
編號:B228994
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 phgH6 [hGH6]
商品貨號 B228994
Designations phgH6 [hGH6]
GenBank Number

X13461

Species Homo sapiens, human
Depositors EE Strehler
Vector
Construct size (kb): 5.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 2.733999967575073
Tissue: leukocyte
Gene product: calmodulin-like [CALML3]
Insert Size (kb) 2.734
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.3, 1.15; PvuII--2.4, 1.1, 0.95, 0.75, 0.35; SacI--2.7 (doublet); SacI/BglI--1.4, 1.15, 0.9, 0.85, 0.65, 0.4; SmaI--3.7, 1.2, 0.75; SmaI/SacI--2.7, 1.2, 0.9, 0.75.
The insert contains the following restriction sites (nt from the 5' end): PstI--106, 752, 1741, 1905, 1998, 2208; PvuII--162, 1077, 2144; SmaI--863, 1569; NotI--953; EcoRI--1099.
Contains a complete open reading frame of 444 nucleotides, with 5' and 3' flanking sequences.
A 0.7 kb SmaI fragment containing the coding region (nt 863 -1569) detects a 2.3 kb mRNA in human erythroleukemia K562 cells. A 1.2 kb SmaI/SacI fragment from the 3' region (nt 1570 - 2738) detects a 1.5 kb mRNA in SV40-transformed human fibroblasts.
The intact insert can be separated from the vector by a SacI/SmaI double digest.
References

Koller M, Strehler EE. Characterization of an intronless human calmodulin-like pseudogene. FEBS Lett. 239: 121-128, 1988. PubMed: 3181418

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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