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pT7.7/hIL-6
pT7.7/hIL-6
規(guī)格:
貨期:
編號(hào):B229542
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pT7.7/hIL-6
商品貨號(hào) B229542
Designations pT7.7/hIL-6
Species Homo sapiens, human
Depositors G Ciliberto
Applications
It is recommended that ATCC 68636 be used instead of ATCC 68507.
Because the host expresses T7 RNA polymerase, and the cDNA is inserted downstream of the phi10 T7 promoter, this strain shows high levels of expression.
Vector
Construct size (kb): 3.924999952316284
Insert
DNA: cDNA
Insert lengths(kb): 1.450000047683716
Gene product: interleukin 6 (interferon, beta 2)(interleukin 6) [IL6]
Insert Size (kb) 1.450
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): XbaI/BamHI--2.5, 0.75, 0.5; HincII--2.8, 0.85; BglII--3.7, HindIII--3.7, BglII/BamHI--2.2, 1.5.
The XbaI site is upstream of the cDNA insert so excision with this enzyme will include some vector sequence.
A new deposit was made for this clone. It is recommended that ATCC 68636 be used instead of ATCC 68507.
Contains 1000 bp of the coding region of mature hIL-6 plus 445 bp of the 3' untranslated sequence.
Because the host expresses T7 RNA polymerase, and the cDNA is inserted downstream of the phi10 T7 promoter, this strain shows high levels of expression. The product is the mature protein and does not require in vitro cleavage of a fusion protein.
References

Gennaro Ciliberto, personal communication

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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